KMID : 0914820030030010026
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Journal of the Korean Gastric Cancer Association 2003 Volume.3 No. 1 p.26 ~ p.32
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Abnormal Fragile Histidine Triad Gene Expression in Gastric Cancer
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Lee Moon-Soo
Kim Tae-Yoon Cho Gyu-Seok Chae Man-Kyu Kim Sung-Yong Baek Moo-Jun Lee Sang-Han Park Kyung-Kyu Kim Chang-Ho Song Ok-Pyung Cho Moo-Sik
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Abstract
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Purpose: Genomic alterations and abnormal expression of the fragile histidine triad (FHIT) gene in gastric cancer were examined to determine whether the FHIT gene is actually a frequent target for alteration during gastric carcinogenesis.
Materials and Methods: To correlate DNA and RNA lesions of the FHIT gene with the effect on FHIT protein expression, in 40 gastric cancers, we investigated the FHIT gene for loss of heterozygisity (LOH), aberrant transcripts, and protein expression.
Results: Allelic loss at D3S1300 was detected in 7 of 38 (19%) informative cases. Aberrant transcripts were observed in 20 of 40 (50%) cases. Significant reduction of FHIT protein expression was observed in 22 of 40 (55%) cases. Aberrant FHIT transcription was shown to be associated with loss of FHIT protein expression. However, aberrent FHIT transcripts themselves were not associated with any clinicopathological parameters, such as age, sex, tumor site, or clinical stage. Moreover, there was no association between the presence of LOH at D3S1300 and the expression of aberrant FHIT transcripts.
Conclusion: The high frequency of aberrant FHIT transcripts, the significant rate of LOH at D3S1300, and the altered expression of the FHIT protein indicate that alterations of the FHIT gene can play an important role in gastric carcinogenesis.
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KEYWORD
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Gastric cancer, FHIT
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